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Mast Cell-mediated Allergic Response Is Suppressed by Sophorae Flos: Inhibition of Src-family Kinase

¹ Konkuk University
² Pusan University
³ Duksung University

^* To whom correspondence should be addressed. E-mail: wahnchoi{at}kku.ac.kr.

	Abstract

Complementary and alternative medicines are considered as a promising direction for the development of anti-allergic therapies in Oriental countries. We screened approximately 100 Oriental herbal medicines for anti-allergic activity. Sophorae flos exhibited the most potent effect on degranulation in antigen-stimulated mast cells. We further investigated the effect of Sophorae flos on the IgE-mediated allergic response in vivo and its mechanism of action in mast cells. Sophorae flos exhibited a significant inhibitory effect on degranulation in antigen-stimulated mast cells with IC50 values of ~31.6 µg/mL (RBL-2H3 mast cells) and ~47.8 µg/mL (bone marrow-derived mast cells). Sophorae flos also suppressed the expression and secretion of TNF- and IL-4 in the cells and IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. Sophorae flos inhibited the activating phosphorylation of Syk and LAT in mast cells. Further downstream, activating phosphorylation of Akt and the prototypic MAP kinases, namely, p38, ERK1/2, and JNK, were also inhibited. These results suggest that Sophorase flos inhibits the Src family kinase-dependent signaling cascades in mast cells and may thus exert anti-allergic activity.

Key Words: Sophorae flos, anti-allergic activity, mast cells, src-family kinase, syk kinase