HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parkinson, P. A. Articles by Leffler, C. W. Search for Related Content PubMed PubMed Citation Articles by Parkinson, P. A. Articles by Leffler, C. W.

---

Original Article

Phospholipase C Activation by Prostacyclin Receptor Agonist in Cerebral Microvascular Smooth Muscle Cells

Laboratory for Research in Neonatal Physiology, Departments of Physiology and Pediatrics, The University of Tennessee, Memphis, Tennessee 38163

The mechanism through which iloprost permits cerebral vasodilation induced by specific stimuli is incompletely understood. Previous study suggests there might be interplay between the adenylyl cyclase and phospholipase C (PLC) systems. Coupling of the prostacyclin receptor with the PLC pathway system was investigated. Iloprost, a stable prostacyclin analog, was used as a prostacyclin receptor agonist. We investigated the effects of iloprost (10^-12–10^-6 M) on inositol 1,4,5-trisphosphate (IP₃) production by piglet cerebrovascular smooth muscle cells in primary culture. Iloprost caused concentration- and time-dependent increases in IP₃ production in control cells and in cells pretreated with LiCl (to prevent further IP₃ metabolism). Iloprost treatment (10^-12 M) of cerebrovascular smooth muscle cells, in the absence and presence of 20 mM LiCl, resulted in 2-fold and 4-fold increases in the formation of IP₃, respectively. In contrast, 10^-10 M to 10^-6 M iloprost, either in the presence or absence of LiCl, induced moderate or no increase in IP₃ formation. Iloprost (10^-10–10^-12 M) strongly stimulated diacylglycerol (DAG) generation, whereas higher concentrations (10^-8 M) did not induce an increase. In conclusion, the results suggest that prostacyclin receptors on cerebromicrovascular smooth muscle can couple to PLC, generating the second messengers, IP₃ and DAG.

This article has been cited by other articles:

				S. N. Cheuvront The Zone Diet Phenomenon: A Closer Look at the Science behind the Claims J. Am. Coll. Nutr., February 1, 2003; 22(1): 9 - 17. [Abstract] [Full Text] [PDF]

				Y. Zhang and C. W. Leffler Compensatory role of NO in cerebral circulation of piglets chronically treated with indomethacin Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2002; 282(2): R400 - R410. [Abstract] [Full Text] [PDF]