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Original Article

Detection of Antigen-Specific Human Serum Proteins Related to the T-Cell Receptor in Infectious Disease and in an Immune Response to Milk Proteins or Chemicals

Colin H. Little^*, George M. Georgiou, Geoffrey Fey, Balachandran Ravindran^§, Joel Levine^¶, Henry Ogedegbe, Harold Yamase and Robert E. Cone^,1

^* Specialist Allergist, Mt. Waverly, Melbourne, Victoria, Australia 3149;
Department of Immunology, Royal Children's Hospital, Melbourne, Australia 3052;
Department of Pathology, University of Connecticut Health Center, Farmington, Connecticut 06030–3105;
^§ Regional Medical Research Center, Bhubaneswar, India;
^¶ Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030–3105

A monoclonal IgG2 antibody, MG3C9–1 A12, was prepared by immunization of mice with human serum Cohn Fraction III proteins enriched for TCR Ca⁺ proteins. MG3C9–1 A12 bound to Mr 28,000, antigen-specific TCR Ca⁺, ß^-, and TCR Ca⁺, ß⁺ serum proteins associated with TGF-ß1, 2. The IgG2 monoclonal antibody also bound to T-lymphocyte proteins but did not bind to B lymphocyte proteins, human albumin, IgM, IgG, IgA, or TGF-ß1, 2, 3 immunogenic peptides. Monoclonal MG3C9–1 A12 detected TCR-related proteins specific for filarial extract, milk proteins, or benzoic acid in the sera of individuals with chronic or asymptomatic filariasis, milk intolerance, or sensitivity to toluene, respectively. TCR-related serum proteins were also detected intracellularly in mononuclear cells in frozen sections of ileum from a patient with milk intolerance and reactive mesenteric lymph nodes from a patient with a gastric ulcer. The results suggest that antigen-specific TCR-related serum proteins may be elevated during an immune response to oral, environmental, or infectious stimuli.

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