| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biochemistry and Molecular Biology, University of Minnesota, Duluth,Minnesota55812
Perinatal copper (Cu) deficiency was studied by offering pregnant Sprague Dawley rats a basal diet low in copper, 0.44 mg/kg, and drinking water containing 0 (-Cu) or 20 (+Cu) mg Cu/L as CuSO4 starting at day 7 of gestation and continuing throughout lactation. To investigate dopamine-ß-monooxygenase (DBM) and tyrosine monooxygenase (TM) in adrenal gland and brain, offspring were weaned at Day 21 to treatments of their respective dams for 9 days. Offspring, 30 days old, of Cu-deficient (-Cu) dams were smaller, anemic, and had biochemical features characteristic of severe Cu deficiency. Adrenal DBM enzyme activity of 30-day-old -Cu rats was 40% higher than Cu-adequate (+Cu) rats and DBM protein levels, estimated by Western immunoblot, were 45% higher. Adrenal DBM mRNA levels of -Cu rats were 108% higher than +Cu rats. Adrenal TM protein levels of -Cu rats were 39% higher than +Cu rats. Hypothalamus DBM activity was significantly higher in -Cu than +Cu rats but no reproducible changes in DBM or TM protein levels could be detected by Western immunoblots. Diet history did not impact adrenal gland or hypothalamus levels of actin as detected on reblotted membranes. However, activity of the cuproenzyme Cu,Zn-superoxide dismutase was 50% lower and 30% lower, respectively, in extracts from rat adrenal gland and hypothalamus of -Cu than +Cu rats, indicating altered Cu status in the tissues studied. These data suggest that Cu deficiency is associated with increased formation of DBM and TM protein levels in adrenal gland. Further research will be required to determine the chemical signal responsible for this induction and if DBM or TM protein levels change in other tissues.
Key Words: rat • dopamine-ß-monooxygenase • tyrosine monooxygenase • copper-deficient
This article has been cited by other articles:
|
|
 |
|
  A. A. Gybina and J. R. Prohaska Fructose-2,6-Bisphosphate Is Lower in Copper Deficient Rat Cerebellum Despite Higher Content of Phosphorylated AMP-Activated Protein Kinase Experimental Biology and Medicine, October 1, 2008; 233(10): 1262 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Pyatskowit and J. R. Prohaska Iron Injection Restores Brain Iron and Hemoglobin Deficits in Perinatal Copper-Deficient Rats J. Nutr., October 1, 2008; 138(10): 1880 - 1886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K Georgieff Nutrition and the developing brain: nutrient priorities and measurement Am. J. Clinical Nutrition, February 1, 2007; 85(2): 614S - 620S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Kuo, A. A. Gybina, J. W. Pyatskowit, J. Gitschier, and J. R. Prohaska Copper Transport Protein (Ctr1) Levels in Mice Are Tissue Specific and Dependent on Copper Status J. Nutr., January 1, 2006; 136(1): 21 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. West and J. R. Prohaska Cu,Zn-Superoxide Dismutase Is Lower and Copper Chaperone CCS Is Higher in Erythrocytes of Copper-Deficient Rats and Mice Experimental Biology and Medicine, September 1, 2004; 229(8): 756 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Gybina and J. R. Prohaska Increased Rat Brain Cytochrome C Correlates with Degree of Perinatal Copper Deficiency Rather than Apoptosis J. Nutr., November 1, 2003; 133(11): 3361 - 3368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Prohaska, J. Geissler, B. Brokate, and M. Broderius Copper, Zinc-Superoxide Dismutase Protein but not mRNA Is Lower in Copper-Deficient Mice and Mice Lacking the Copper Chaperone for Superoxide Dismutase Experimental Biology and Medicine, September 1, 2003; 228(8): 959 - 966. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
