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Experimental Biology and Medicine 226:577-580 (2001)
© 2001 Society for Experimental Biology and Medicine

ORIGINAL ARTICLE

Black Tea Extract, Thearubigin Fraction, Counteracts the Effect of Tetanus Toxin in Mice

Eiki Satoh*, Toshiaki Ishii*, Yoshio Shimizu{dagger}, Sin-ichi Sawamura{ddagger} and Masakazu Nishimura*,1

* Department of Pharmacology;
{dagger} Cooperative Research Centre, University of Obihiro School of Veterinary Medicine, Obihiro 080–8555, Japan;
{ddagger} Ito en Ltd., Shibuya ward, Tokyo151–0071,Japan

The aim of this study was to find an inactivating substance for tetanus toxin in natural foodstuff. Tetanus toxin (4 µg/ml) abolished indirect twitches in in vitro mouse phrenic nerve-diaphragm preparations within 2.5 hr. Hot water infusion of black tea mixed with tetanus toxin blocked the inhibitory effect of the toxin. Mixing the toxin with thearubigin fraction extracted from black tea infusion produced an identical result. Furthermore, thearubigin fraction mixed with the toxin protected against the in vivo paralytic effect of the toxin. Thearubigin fraction had no protective effect on other toxins, such as tetrodotoxin and saxitoxin. The specific binding of [125I]tetanus toxin to rat cerebrocortical synaptosomes was inhibited by mixing iodinated toxin with thearubigin fraction. These results imply that thearubigin fraction counteracts the effect of tetanus toxin by binding with toxin, and also suggest that this fraction may be able to apply for prophylaxis of tetanus.

Key Words: tetanus toxin • thearubigin fraction • diaphragm • synaptosome • mouse






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