EBM Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woller, M.
Right arrow Articles by Waechter-Brulla, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woller, M.
Right arrow Articles by Waechter-Brulla, D.
Experimental Biology and Medicine 226:591-596 (2001)
© 2001 Society for Experimental Biology and Medicine

ORIGINAL ARTICLE

Leptin Stimulates Gonadotropin Releasing Hormone Release From Cultured Intact Hemihypothalami and Enzymatically Dispersed Neurons

Michael Woller,1, Shyla Tessmer, Don Neff, Aude Ada Nguema, Bart Van Roo and Daryle Waechter-Brulla

Department of Biological Sciences, University of Wisconsin-Whitewater, Whitewater,Wisconsin53190

Leptin is a peptide released by adipocytes that has profound effects on central regulation of body metabolism. The present study represents an investigation into leptin effects on hypothalamic control of reproductive function, specifically on GnRH release. Adult male rats (gonadectomized or sham-operated) were used as donors of hypothalamic tissue that was used as intact hemihypothalami or as enzymatically dispersed hemihypothalami in a perifusion culture system. Continuous samples were collected at 10-min intervals for 8 to 10 hr and were assayed to measure temporal changes in GnRH release in response to various doses of leptin infused into the perifusion chambers. Leptin at the highest dose (10-8 M) resulted in consistent and significant stimulation of GnRH release. There were no effects of treatment for surgical preparation (gonadectomy versus sham) or tissue preparation (intact versus dispersed hemihypothalami). The results of this study support the hypothesis that leptin plays a direct stimulatory role in the regulation of GnRH release. This study describes an important step in our understanding of the mechanism that connects changes in basal metabolism with reproductive function. These results indicate an intact interneuronal network is unnecessary for these leptin effects, but does not exclude a role for interneuronal networks in this regulatory pathway.

Key Words: GnRH • leptin • hypothalamic tissue culture




This article has been cited by other articles:


Home page
 EndocrinologyHome page
J. H. Quennell, A. C. Mulligan, A. Tups, X. Liu, S. J. Phipps, C. J. Kemp, A. E. Herbison, D. R. Grattan, and G. M. Anderson
Leptin Indirectly Regulates Gonadotropin-Releasing Hormone Neuronal Function
Endocrinology, June 1, 2009; 150(6): 2805 - 2812.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. C. Russo, S. Metaxas, K. Kobayashi, M. Harris, and G. A. Werther
Antiapoptotic Effects of Leptin in Human Neuroblastoma Cells
Endocrinology, September 1, 2004; 145(9): 4103 - 4112.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D.A. Zieba, M. Amstalden, S. Morton, M.N. Maciel, D.H. Keisler, and G.L. Williams
Regulatory Roles of Leptin at the Hypothalamic-Hypophyseal Axis Before and after Sexual Maturation in Cattle
Biol Reprod, September 1, 2004; 71(3): 804 - 812.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
M. J. Woller, S. Meyer, A. Ada-Nguema, and D. Waechter-Brulla
Dissecting Autocrine Effects on Pulsatile Release of Gonadotropin-Releasing Hormone in Cultured Rat Hypothalamic Tissue
Experimental Biology and Medicine, January 1, 2004; 229(1): 56 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M.N. Maciel, D.A. Zieba, M. Amstalden, D.H. Keisler, J.P. Neves, and G.L. Williams
Leptin Prevents Fasting-Mediated Reductions in Pulsatile Secretion of Luteinizing Hormone and Enhances Its Gonadotropin-Releasing Hormone-Mediated Release in Heifers
Biol Reprod, January 1, 2004; 70(1): 229 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Amstalden, D.A. Zieba, J.F. Edwards, P.G. Harms, T.H. Welsh Jr., R.L. Stanko, and G.L. Williams
Leptin Acts at the Bovine Adenohypophysis to Enhance Basal and Gonadotropin-Releasing Hormone-Mediated Release of Luteinizing Hormone: Differential Effects Are Dependent upon Nutritional History
Biol Reprod, November 1, 2003; 69(5): 1539 - 1544.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. E. Wilson, J. Fisher, K. Chikazawa, R. Yoda, A. Legendre, D. Mook, and K. G. Gould
Leptin Administration Increases Nocturnal Concentrations of Luteinizing Hormone and Growth Hormone in Juvenile Female Rhesus Monkeys
J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4874 - 4883.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Burcelin, B. Thorens, M. Glauser, R. C. Gaillard, and F. P. Pralong
Gonadotropin-Releasing Hormone Secretion from Hypothalamic Neurons: Stimulation by Insulin and Potentiation by Leptin
Endocrinology, October 1, 2003; 144(10): 4484 - 4491.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. J. Cunningham, J. M. Scarlett, and R. A. Steiner
Cloning and Distribution of Galanin-Like Peptide mRNA in the Hypothalamus and Pituitary of the Macaque
Endocrinology, March 1, 2002; 143(3): 755 - 763.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Society for Experimental Biology and Medicine.