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Experimental Biology and Medicine 227:57-62 (2002)
© 2002 Society for Experimental Biology and Medicine

ORIGINAL ARTICLE

BMP-4 and BMP-6 Involvement in the Osteogenic Properties of the HeLa Cell Line

Iwona Ewa Kochanowska*, Krzysztof Wlodarski{dagger}, Andrzej Wojtowicz§, Agnieszka Kinsner{ddagger} and Kazimierz Ostrowski1,{dagger}

* Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland; and Departments of
{dagger} Histology and
{ddagger} Transplantology and
§ Dental Surgery Clinic, Medical Academy of Warsaw, 02-004 Warsaw, Poland

The heterotopically induced ossicles are used in our research on bone tissue. The ossicles are formed in the thigh muscle of BALB/c mice under the influence of injected suspension of 3 x 106 HeLa cells. We postulate that the mechanism of bone induction is based on the secretion of bone morphogenetic proteins BMP-4 and BMP-6 by the grafted HeLa cells. This was proved by the use of specific immunohistochemical reaction and Western blots of conditioned culture medium. It seems that HeLa cells secrete BMPs continuously into the culture medium, even without contact with the mice muscle tissue, were induction of bone tissue is observed.

Key Words: bone induction • HeLa cells • bone morphogenetic proteins BMP-4 and BMP-6




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[Abstract] [Full Text] [PDF]


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