| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,2
* Research Service, Department of Veterans Affairs Medical Center, Omaha, NE 68105; and
Pulmonary and Critical Care Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, 68198
Relaxin is an insulin-like serum protein secreted during pregnancy and found in many tissues, including the lung. Relaxin is reported to stimulate epithelial cell proliferation, but the effects of relaxin on airway epithelium are unknown. We tested the hypothesis that relaxin would stimulate the increased migration of bronchial epithelial cells (BEC) in response to wounding. Using monolayers of BEC in a wound-healing model, relaxin augmented wound closure with maximal closure occurring at 12 hr (1 µM). Unlike cytokines, relaxin did not stimulate increased BEC interleukin-8 (IL-8) release. Relaxin caused a significant stimulation of ciliary beat frequency (CBF) in BEC. Because protein kinase (PKA) activation increases CBF and relaxin can elevate intracellular cAMP levels, we measured PKA activity in BEC treated with relaxin. Relaxin increased PKA activity 3-4 fold by approximately 4 hr, with a return to baseline levels by 8–10 hr. Relaxin-stimulated PKA activity differs temporally from the rapid (1 hr) ß-adrenergic activation of PKA in BEC. These data suggest that relaxin augments epithelial repair by increasing airway cell migration and CBF via PKA-dependent mechanisms.
Key Words: airway epithelial • wound healing • relaxin • cAMP • cilia
This article has been cited by other articles:
|
|
 |
|
  C. S. Samuel, S. G. Royce, M. D. Burton, C. Zhao, G. W. Tregear, and M. L. K. Tang Relaxin Plays an Important Role in the Regulation of Airway Structure and Function Endocrinology, September 1, 2007; 148(9): 4259 - 4266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hombach-Klonisch, J. Bialek, B. Trojanowicz, E. Weber, H.-J. Holzhausen, J. D. Silvertown, A. J. Summerlee, H. Dralle, C. Hoang-Vu, and T. Klonisch Relaxin Enhances the Oncogenic Potential of Human Thyroid Carcinoma Cells Am. J. Pathol., August 1, 2006; 169(2): 617 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Allen-Gipson, J. Wong, J. R. Spurzem, J. H. Sisson, and T. A. Wyatt Adenosine A2A receptors promote adenosine-stimulated wound healing in bronchial epithelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L849 - L855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Figueiredo, A. L. Mui, C. C. Nelson, and M. E. Cox Relaxin Stimulates Leukocyte Adhesion and Migration through a Relaxin Receptor LGR7-dependent Mechanism J. Biol. Chem., February 10, 2006; 281(6): 3030 - 3039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mookerjee, N. R. Solly, S. G. Royce, G. W. Tregear, C. S. Samuel, and M. L. K. Tang Endogenous Relaxin Regulates Collagen Deposition in an Animal Model of Allergic Airway Disease Endocrinology, February 1, 2006; 147(2): 754 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Boxall, S. T. Holgate, and D. E. Davies The contribution of transforming growth factor-{beta} and epidermal growth factor signalling to airway remodelling in chronic asthma Eur. Respir. J., January 1, 2006; 27(1): 208 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. D. Sherwood Relaxin's Physiological Roles and Other Diverse Actions Endocr. Rev., April 1, 2004; 25(2): 205 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Silvertown, B. J. Geddes, and A. J. S. Summerlee Adenovirus-Mediated Expression of Human Prorelaxin Promotes the Invasive Potential of Canine Mammary Cancer Cells Endocrinology, August 1, 2003; 144(8): 3683 - 3691. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
