| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Influenza Research Center, Respiratory Pathogens Research Unit, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030
Recent studies indicate that respiratory syncytial virus (RSV), like influenza, causes significant morbidity and mortality among elderly persons. There are currently no animal models to study the effects of aging on RSV disease and immunity. This manuscript provides an initial description of such a model. Aged and young BALB/c mice (22–24 and 2–4 months, respectively) were infected with 104 TCID50 of RSV A2. RSV was detected by culture in lung and nose wash specimens obtained 4–6 days following infection at a slightly higher titer in old mice in comparison with young mice. RT-PCR assay detected RSV in the lungs and nose washes of all mice on 4, 8, and 21 days postinoculation, with only a slightly less frequency in young mice. Splenic lymphocytes from old mice exhibited significantly lower RSV-specific MHC class I-restricted CD8+ CTL responses (P < 0.01–0001), and reduced IFN-production (P < 0.03) than young mice. Conversely, IL-4 production was somewhat elevated in old mice. These results demonstrate diminished RSV virus-specific CD8+CTL responses and IFN-
production in old mice in comparison with young. It is speculated that the deficient RSV-specific CTL responses may account for the increased morbidity and mortality from RSV infections in elderly persons. Although detailed histopathological, virological, and immunological analyses are incomplete at present, the old BALB/c RSV infection model described provides an opportunity to evaluate the role of CD8+CTL and cytokines in RSV disease in aging.
Key Words: respiratory syncytial virus • CD8+ CTL • IFN- • aging
This article has been cited by other articles:
|
|
 |
|
  M. Darniot, C. Pitoiset, T. Petrella, S. Aho, P. Pothier, and C. Manoha Age-Associated Aggravation of Clinical Disease after Primary Metapneumovirus Infection of BALB/c Mice J. Virol., April 1, 2009; 83(7): 3323 - 3332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Baas, A. Roberts, T. H. Teal, L. Vogel, J. Chen, T. M. Tumpey, M. G. Katze, and K. Subbarao Genomic Analysis Reveals Age-Dependent Innate Immune Responses to Severe Acute Respiratory Syndrome Coronavirus J. Virol., October 1, 2008; 82(19): 9465 - 9476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Liu and Y. Kimura Local immune response to respiratory syncytial virus infection is diminished in senescence-accelerated mice J. Gen. Virol., September 1, 2007; 88(9): 2552 - 2558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-E. Hamelin, K. Yim, K. H. Kuhn, R. P. Cragin, M. Boukhvalova, J. C. G. Blanco, G. A. Prince, and G. Boivin Pathogenesis of Human Metapneumovirus Lung Infection in BALB/c Mice and Cotton Rats J. Virol., July 15, 2005; 79(14): 8894 - 8903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Roberts, C. Paddock, L. Vogel, E. Butler, S. Zaki, and K. Subbarao Aged BALB/c Mice as a Model for Increased Severity of Severe Acute Respiratory Syndrome in Elderly Humans J. Virol., May 1, 2005; 79(9): 5833 - 5838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hagiwara, J. R. McGhee, K. Fujihashi, R. Kobayashi, N. Yoshino, K. Kataoka, Y. Etani, M.-N. Kweon, S. Tamura, T. Kurata, et al. Protective Mucosal Immunity in Aging Is Associated with Functional CD4+ T Cells in Nasopharyngeal-Associated Lymphoreticular Tissue J. Immunol., February 15, 2003; 170(4): 1754 - 1762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Curtis, M. G. Ottolini, D. D. Porter, and G. A. Prince Age-Dependent Replication of Respiratory Syncytial Virus in the Cotton Rat Experimental Biology and Medicine, October 1, 2002; 227(9): 799 - 802. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
