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SUPPLEMENT. FOOD SAFETY CONCERNS OF VEROTOXIN-PRODUCING ESCHERICHIA COLI

Verotoxin-Producing Escherichia coli in Culled Beef Cows Grazing Rangeland Forages

Hussein S. Hussein^*,1, Brandolyn H. Thran^*, Mark R. Hall^*,, William G. Kvasnicka^* and Rodney C. Torell

^* Department of Animal Biotechnology,
Department of Microbiology, and
Nevada Cooperative Extension, University of Nevada, Reno, Nevada 89557

Abstract

The objective of this study was to assess prevalence of verotoxin-producing Escherichia coli (VTEC) in culled beef cows at the time of shipping to slaughter. Feces were collected from 82 cows on eight Nevada ranches during fall and winter (from September to January) after grazing rangeland forages. A random sample (n = 154) of potential VTEC isolates were tested for verotoxicity and were screened for the presence (polymerase chain reaction) and expression (VTEC-reversed passive latex agglutination assay) of the toxin genes (i.e., VT1 and VT2). Seventeen isolates from four ranches were VTEC. Of these, four had the VT1 gene, five had the VT2 gene, seven had both genes, and one did not have either gene despite its toxicity to Vero cells. Except for one isolate (i.e., untypeable that reacted with VT1-latex beads without having VT1 gene), the genotype and phenotype data of the VTEC isolates matched. Another isolate (O8:H^- [nonmotile]) was verotoxic, but neither had nor expressed the toxin genes. Of the 17 isolates, four (from one cow) were O157:H7, 11 (from five cows on three ranches) were non-O157:H7 (two O8:H^-, three O105:H^-, three O116:H^-, and three O141:H^-), and two were untypeable. Because some of these VTEC serotypes (i.e., O8:H^-, O141:H^-, and O157:H7) are known to cause human illnesses, it is beneficial to identify VTEC-positive cows before slaughter. This is a critical step in any pre- or post-harvest strategy to minimize the risk of beef contamination with such pathogens.

Key Words: Escherichia coli • beef cattle • culled cows • verotoxins • food-borne pathogens

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