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HEME OXYGENASE

Generation of Heme Oxygenase-1-Transgenic Rats

C. Braudeau^*, D. Bouchet^*, C. Toquet, L. Tesson^*, S. Ménoret^*, S. Iyer, C. Laboisse, D. Willis, A. Jarry, R. Buelow, I. Anegon^1,2,* and C. Chauveau^1,*

^* Institut National de la Santé Et de la Recherche Médicale (INSERM) U437/Institut de Transplantation Et de Recherche en Transplantation (ITERT) and
Service d’Anatomie Pathologique, 44093 Nantes Cedex 01, France;
University College of London, London WC1E 6BT, UK; and
SangStat Corporation, Fremont, California 94555

Abstract

Heme oxygenase-1 (HO-1) expression protects cells from a variety of cellular insults and inhibits inflammation. However, its role in the regulation of immune responses has not yet been clearly established. We generated HO-1 transgenic rats to directly test the impact of HO-1 on the different immune mechanisms. To temporally control the expression of HO-1, we used a one-plasmid tetracycline (tet)-inducible system. This plasmid contains the H-2K^b promoter, which transcribes the tet transactivator (tTA) and expression of a human HO-1 cDNA is obtained in the absence of tetracycline. The DNA construct was microinjected into one-cell rat embryos and mothers and pups were maintained with tetracycline. Eight transgenic founders were obtained. Analysis of transgene expression in the absence of tet showed that 2 lines (12.4 and 12.6) expressed HO-1 mRNA in several organs (as detected by reverse transcription polymerase chain reaction) and at the protein level only in the thymus. Expression levels of transgene-derived HO-1 increased after withdrawal of tet compared with transgenic rats maintained with tet, as detected by analysis of mRNA levels by quantitative real-time reverse transcription polymerase chain reaction. Gross examination and histopathological analysis of several organs in both lines showed no anomalies. Thymocytes and splenocytes of both lines showed normal cell subpopulations and allogeneic proliferation compared with controls. Systemic immune responses against cognate antigens were normal in both lines, as evaluated by the proliferation of lymph node cells and the production of antibodies against keyhole limpet hemocyanin after immunization. Animals from line 12.6 rejected transplanted allogeneic hearts with the same kinetics as controls. In conclusion, short-term induction of HO-1 overexpression did not modify immune responses compared to those of control non-transgenic animals.

Key Words: HO-1 • transgenic rats • immune response • transplantation

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