| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Laboratory Sciences, School of Health Sciences, Faculty of Medicine, Kanazawa University, Kanazawa 920-0942, Japan and
Department of Pediatrics and Angiogenesis and Vascular Development, Kanazawa University Graduate School of Medical Science, 920-8641, Japan
Abstract
Monocytes play key roles both in innate and adaptive antigen-specific immunity and they constitute critical components of the immune responses. Although most of the monocyte-derived cytokines exhibit proinflammatory functions in vivo, heme oxygenase-1 (HO-1), an inducible heme-degrading enzyme, exerts potent anti-inflammatory effect through production of carbon monoxide and bilirubin. We compared HO-1 production by monocytes in vivo in various acute inflammatory illnesses and in normal controls. Freshly isolated monocytes produced little HO-1 as detected by immunohistochemistry, but it was rapidly induced in vitro upon stimulation. HO-1 production by monocytes was selective because it was not induced in other leukocyte populations, including granulocytes and lymphocytes. Monocytes from acute inflammatory illnesses, such as Kawasaki disease and acute infectious diseases, viral or bacterial, produced significant levels of HO-1, as detected by flow cytometry, immunohistochemistry, and reverse transcription polymerase chain reaction. Quantitative analysis of HO-1 mRNA expression by real-time polymerase chain reaction revealed that monocytes from controls exhibited low, but significant levels of HO-1 mRNA, indicating that circulating monocytes produce HO-1 constantly, in response to basal level of oxidative stress encountered daily. Significantly elevated HO-1 mRNA levels seen in acute inflammatory illnesses suggest that monocyte HO-1 production serve as potent anti-inflammatory agent to control excessive cell or tissue injury in the presence of oxidative stress and cytokinemia.
Key Words: inflammation • heme oxygenase • oxidative stress
This article has been cited by other articles:
|
|
 |
|
  F. Gueler, J.-K. Park, S. Rong, T. Kirsch, C. Lindschau, W. Zheng, M. Elger, A. Fiebeler, D. Fliser, F. C. Luft, et al. Statins Attenuate Ischemia-Reperfusion Injury by Inducing Heme Oxygenase-1 in Infiltrating Macrophages Am. J. Pathol., April 1, 2007; 170(4): 1192 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Drechsler, A. Dolganiuc, O. Norkina, L. Romics, W. Li, K. Kodys, F. H. Bach, P. Mandrekar, and G. Szabo Heme Oxygenase-1 Mediates the Anti-Inflammatory Effects of Acute Alcohol on IL-10 Induction Involving p38 MAPK Activation in Monocytes J. Immunol., August 15, 2006; 177(4): 2592 - 2600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Yasuda, S Okinaga, M Yamaya, T Ohrui, M Higuchi, M Shinkawa, S Itabashi, K Nakayama, M Asada, A Kikuchi, et al. Association of susceptibility to the development of pneumonia in the older Japanese population with haem oxygenase-1 gene promoter polymorphism. J. Med. Genet., April 1, 2006; 43(4): e17 - e17. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wu and R. Wang Carbon Monoxide: Endogenous Production, Physiological Functions, and Pharmacological Applications Pharmacol. Rev., December 1, 2005; 57(4): 585 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Reade, J. L. Millo, J. D. Young, and C. A. R. Boyd Nitric oxide synthase is downregulated, while haem oxygenase is increased, in patients with septic shock Br. J. Anaesth., April 1, 2005; 94(4): 468 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. GIL, G. MARTINEZ, R. TAPANES, O. CASTRO, D. GONZALEZ, L. BERNARDO, S. VAZQUEZ, G. KOURI, and M. G. GUZMAN OXIDATIVE STRESS IN ADULT DENGUE PATIENTS Am J Trop Med Hyg, November 1, 2004; 71(5): 652 - 657. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
