EBM Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McLaughlin, B. E.
Right arrow Articles by Brien, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McLaughlin, B. E.
Right arrow Articles by Brien, J. F.
Experimental Biology and Medicine 228:564-567 (2003)
© 2003 Society for Experimental Biology and Medicine

HEME OXYGENASE

Heme Oxygenase Expression in Selected Regions of Term Human Placenta

Brian E. McLaughlin*, Gendie E. Lash*,{dagger}, Graeme N. Smith*,{dagger},{ddagger}, Gerald S. Marks*, Kanji Nakatsu*, Charles H. Graham*,{dagger} and James F. Brien*,1

* Departments of Pharmacology and Toxicology,
{dagger} Anatomy and Cell Biology, and
{ddagger} Obstetrics and Gynaecology, Faculty of Health Sciences, Queen’s University, Kingston, Ontario, Canada K7L 3N6

Abstract

Carbon monoxide (CO), formed during heme oxygenase (HO)-catalyzed oxidation of heme, has been proposed to play a complementary role with nitric oxide in the regulation of placental hemodynamics. The objective of this study was to elucidate HO enzymatic activity and HO-1 (inducible) and HO-2 (constitutive) protein content in the microsomal subcellular fraction of homogenate of selected regions of placenta from normotensive and mild pre-eclamptic pregnancies. HO enzymatic activity was measured under optimized conditions by gas chromatography using CO formation as an index of activity, and HO-1 and HO-2 protein content were determined by Western immunoblot analysis. Microsomal HO activity in each of the four placental regions was not different between normotensive and mild pre-eclamptic pregnancies. Microsomal HO-2 protein content was not different between normotensive and mild pre-eclamptic pregnancies, whereas there was increased expression of microsomal HO-1 protein in chorionic villi and fetal membranes from pre-eclamptic pregnancy compared with normotensive pregnancy. Microsomal HO enzymatic activity correlated with HO-2, but not HO-1, protein content.

Key Words: heme oxygenase activity • carbon monoxide formation • heme oxygenase protein • term human placenta • pre-eclampsia




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
H. Zhao, R. J. Wong, T. C. Doyle, N. Nayak, H. J. Vreman, C. H. Contag, and D. K. Stevenson
Regulation of Maternal and Fetal Hemodynamics by Heme Oxygenase in Mice
Biol Reprod, April 1, 2008; 78(4): 744 - 751.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
S. Zhao, Y. Zhang, Y. Gu, D. F. Lewis, and Y. Wang
Heme Oxygenase-1 Mediates Up-Regulation of Adhesion Molecule Expression Induced by Peroxynitrite in Endothelial Cells
Reproductive Sciences, October 1, 2004; 11(7): 465 - 471.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. D. Appleton, G. E. Lash, G. S. Marks, K. Nakatsu, J. F. Brien, G. N. Smith, and C. H. Graham
Effect of glucose and oxygen deprivation on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2003; 285(6): R1453 - R1460.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Society for Experimental Biology and Medicine.