HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Prather, R. S. Articles by Green, J. A. Search for Related Content PubMed PubMed Citation Articles by Prather, R. S. Articles by Green, J. A.

---

MINIREVIEW

Nuclear Remodeling and Reprogramming in Transgenic Pig Production

Randall S. Prather^*,1, Peter Sutovsky^*, and Jonathan A. Green^*

^* Departments of Animal Science and Obstetrics and Gynecology, and Food for the 21st Century Program, College of Agriculture, Food and Natural Resources, University of Missouri at Columbia, Columbia, Missouri 65211

To whom requests for reprints should be addressed at ¹ E125D Animal Science Research Center, 920 East Campus Drive, University of Missouri at Columbia, Columbia, MO 65211–5300. E-mail: PratherR{at}Missouri.Edu

The manufacture of pigs with modifications to specific chromosomal regions requires that the modification first be made in somatic cells. The modified cells can then be used as donors for nuclear transfer (NT) in an attempt to clone that cell into a newborn animal. Unfortunately the procedures are inefficient and sometimes lead to animals that are abnormal. The cause of these abnormalities is likely established during the first cell cycle after the NT. Either the donor cell was abnormal or the oocyte cytoplasm was unable to adequately remodel the donor nucleus such that it was structured similar to the pronucleus of a zygote. A better understanding of chromatin remodeling and subsequent developmental gene expression will provide clues as to how procedures can be modified to generate fertile animals more efficiently.

Key Words: cloning • nuclear transfer • transgenic • pig

This article has been cited by other articles:

				S.M. Mitalipov, Q. Zhou, J.A. Byrne, W.Z. Ji, R.B. Norgren, and D.P. Wolf Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling Hum. Reprod., August 1, 2007; 22(8): 2232 - 2242. [Abstract] [Full Text] [PDF]