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ORIGINAL RESEARCH ARTICLE

Hypoxic Rise in Cytosolic Calcium and Renal Proximal Tubule Injury Mediated by a Nickel-Sensitive Pathway

M. Barac-Nieto, A. Constantinescu, M. H. Pina-Benabou^* and R. Rozental^*,1

^* Department of Cell Biology and Anatomy and Departments of Obstetrics and Anesthesiology, New York Medical College, Valhalla, New York 10595; Department of Physiology, Kuwait University, Kuwait; and Children’s Hospital, Hollywood, Florida 33021

To whom requests for reprints should be addressed at ¹ Department of Cell Biology and Anatomy, BSB Room A21, Departments of Obstetrics and Anesthesiology, New York Medical College, Valhalla, NY 10595. E-mail: r_rozental{at}nymc.edu

In the kidney, cell injury resulting from ischemia and hypoxia is thought to be due, in part, to increased cytosolic Ca²⁺ levels, [Ca²⁺]i, leading to activation of lytic enzymes, cell dysfunction, and necrosis. We report evidence of a progressive and exponential increase in [Ca²⁺]i (from 245 ± 10 to 975 ± 100 nM at 45 mins), cell permeabilization and propidium iodide (PI) staining of the nucleus, and partial loss of cell transport functions such as Na⁺-gradient–dependent uptakes of ¹⁴C-alpha-methylglucopyranoside and inorganic phosphate (³²Pi) in proximal convoluted tubules of adult rabbits subjected to hypoxia. The rise in [Ca²⁺]i depended on the presence of extracellular [Ca²⁺] and could be blocked by 50 µM Ni²⁺but not by verapamil (100 µM). Presence of 50 µM Ni²⁺ also reduced the hypoxia-induced morphological and functional injuries. We also used HEK 293 cells, a kidney cell line, incubated in media without glucose and exposed for 3.5 hrs to 1% O₂–5% CO₂ and then returned to glucose-containing media for another 3.5 hrs in an air–5% CO₂ atmosphere and finally exposed for 1 min to media containing 1 µM PI. NiCl₂ (50 µM) or pentobarbital (300 µM) more than phenobarbital (1.5 mM), when present in the incubation medium during both the hypoxic and the reoxygenation periods, induced significant (P < 0.001) reductions in the number of cell nuclei stained with PI, similar to their relative potency as inhibitors of T channels. Our findings indicate that hypoxia-induced alterations in calcium level and subsequent cell injury in the proximal convoluted tubule and in HEK cells involve a nickel-sensitive and dihydropyridine insensitive pathway or channel.

Key Words: hypoxia • calcium • nickel • kidney • HEK cells • pentobarbital • phenobarbital

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