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ORIGINAL RESEARCH ARTICLE

Senescent B Lymphopoiesis Is Balanced in Suppressive Homeostasis: Decrease in Interleukin-7 and Transforming Growth Factor-ß Levels in Stromal Cells of Senescence-Accelerated Mice

Isao Tsuboi^*,, Kohji Morimoto^*, Yoko Hirabayashi^*, Guang-Xun Li^*, Shin Aizawa, Kazuhiro J. Mori, Jun Kanno^* and Tohru Inoue^,1

^* Division of Cellular and Molecular Toxicology, National Institute of Health Sciences, Tokyo 158–8501, Japan; Department of Anatomy and Developmental Biology, Nihon University School of Medicine, Tokyo 173–8610, Japan; Department of Molecular and Cellular Biology, Faculty of Science, Niigata University, Niigata 950–2181, Japan; and Center for Biological Safety and Research, National Institute of Health Sciences, Tokyo 158–8501, Japan

To whom requests for reprints should be addressed at ¹ Center for Biological Safety and Research, National Institute of Health Sciences, 1-18-1 Kamiyohga, Setagayaku, Tokyo 158–8501, Japan. E-mail: tohru{at}nihs.go.jp

The suppression of the B cell population during senescence has been considered to be due to the suppression of interleukin-7 (IL-7) production and responsiveness to IL-7; however, the upregulation of transforming growth factor-ß (TGF-ß) was found to contribute to B cell suppression.To investigate the mechanism of this suppression based on the interrelationship between IL-7 and TGF-ß during senescence, senescence-accelerated mice (SAMs), the mouse model of aging, were used in this study to elucidate the mechanisms of B lymphopoietic suppression during aging. Similar to regular senescent mice, SAMs showed a decrease in the number of IL-7–responding B cell progenitors (i.e., colony-forming unit pre-B [CFU-pre-B] cells in the femoral bone marrow [BM]). A co-culture system of B lymphocytes and stromal cells that the authors established showed a significantly lower number of CFU-pre-B cells harvested when BM cells were co-cultured with senescent stromal cells than when they were co-cultured with young stromal cells. Interestingly, cells harvested from a senescent stroma and those from the control culture without stromal cells were higher in number than those harvested from a young stroma, thereby implying that an altered senescent stromal cell is unable to maintain self-renewal of the stem cell compartment. Because TGF-ß is supposed to suppress the proliferative capacity of pro-B/pre-B cells, we added a neutralizing anti-TGF-ß antibody to the co-culture system with a pro-B/pre-B cell-rich population to determine whether such suppression may be rescued. However, unexpectedly, any rescue was not observed and the number of CFU-pre-B cells remained unchanged when BM cells were co-cultured with senescent stromal cells compared with the co-culture with young stromal cells, which essentially showed an increase in the number of CFU-pre-B cells (P < 0.001 in 5 µg/ml). Furthermore, TGF-ß protein level in the supernatant of cultured senescent stroma cells was evaluated by enzyme-linked immunoabsorbent assay, but surprisingly, it was found that TGF-ß concentration was significantly lower than that of cultured young stromal cells. Thus, TGF-ß activity was assumed to decline particularly in a senescent stroma, which means a distinct difference between the senescent suppression of B lymphopoiesis and secondary B lymphocytopenia. Concerning proliferative signaling, on the other hand, the level of IL-7 gene expression in cells from freshly isolated BM decreased significantly with age. Therefore, the acceleration of proliferative signaling and the deceleration of suppressive signaling may both be altered and weakened in a senescent stroma (i.e., homeosupression).

Key Words: aging • B-lymphopoiesis • interleukin-7 • transforming growth factor-ß • • senescence-accelerated mice • homeosuppression

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				A. Minami, I. Tsuboi, T. Harada, T. Fukumoto, M. Hiramoto, M. Koshinaga, Y. Hirabayashi, J. Kanno, T. Inoue, and S. Aizawa Inflammatory Biomarker, Neopterin, Suppresses B Lymphopoiesis for Possible Facilitation of Granulocyte Responses, Which Is Severely Altered in Age-Related Stromal-Cell-Impaired Mice, SCI/SAM Experimental Biology and Medicine, January 1, 2007; 232(1): 134 - 145. [Abstract] [Full Text] [PDF]