HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Crossgrove, J. S. Articles by Zheng, W. Search for Related Content PubMed PubMed Citation Articles by Crossgrove, J. S. Articles by Zheng, W.

---

ORIGINAL RESEARCH ARTICLE

The Choroid Plexus Removes ß-Amyloid from Brain Cerebrospinal Fluid

School of Health Sciences, Purdue University, West Lafayette, Indiana 47907

To whom requests for reprints should be addressed at ¹ School of Health Sciences, Purdue University, 550 Stadium Mall Drive, CIVL-1163D, West Lafayette, IN 47907. E-mail: wzheng{at}purdue.edu

ß-Amyloid (Aß) concentration in the cerebrospinal fluid (CSF) of the brain may be regulated by the choroid plexus, which forms a barrier between blood and brain CSF. Aß uptake from CSF was determined as its volume of distribution (V_D) into isolated rat choroid plexus tissue. The V_D of [¹²⁵I]Aß_1–40 was corrected by subtraction of the V_D of [¹⁴C]sucrose, a marker for extracellular space and diffusion. Aß uptake into choroid plexus was time and temperature dependent. Uptake of [¹²⁵I]Aß was saturable. Aß uptake was not affected by addition of transthyretin or apolipoprotein E3. In studies with primary culture monolayers of choroidal epithelial cells in Transwells, Aß permeability across cells, corrected by [¹⁴C]sucrose, was greater from the CSF-facing membrane than from the blood-facing membrane. Similarly, cellular accumulation of [¹²⁵I]Aß was concentrative from both directions and was greater from the CSF-facing membrane, suggesting a bias for efflux. Overall, these results suggest the choroid plexus selectively cleanses Aß from the CSF by an undetermined mechanism(s), potentially reducing Aß from normal brains and the brains of Alzheimer’s disease patients.

Key Words: choroid plexus • ß-amyloid • cerebrospinal fluid

This article has been cited by other articles:

				Q. Xu, A. Bernardo, D. Walker, T. Kanegawa, R. W. Mahley, and Y. Huang Profile and Regulation of Apolipoprotein E (ApoE) Expression in the CNS in Mice with Targeting of Green Fluorescent Protein Gene to the ApoE Locus. J. Neurosci., May 10, 2006; 26(19): 4985 - 4994. [Abstract] [Full Text] [PDF]

				F. Naftolin Another Crack in the Edifice of the Brain-Blood Interface. Experimental Biology and Medicine, May 1, 2006; 231(5): 489 - 489. [Full Text] [PDF]