HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murdoch, W. J. Articles by Alexander, B. M. Search for Related Content PubMed PubMed Citation Articles by Murdoch, W. J. Articles by Alexander, B. M.

---

ORIGINAL RESEARCH ARTICLE

A BRIEF COMMUNICATION

DNA Damages in Ovarian Surface Epithelial Cells of Ovulatory Hens

Department of Animal Science and Reproductive Biology Program, University of Wyoming, Laramie, Wyoming 82071

To whom requests for reprints should be addressed at ¹ Department of Animal Science 3684, 1000 E. University Avenue, University of Wyoming, Laramie, WY 82071. E-mail: wmurdoch{at}uwyo.edu

A cause-effect relationship between ovulation and common (surface) epithelial ovarian cancer has been suspected for many years. The ovarian surface epithelium apparently becomes exposed to genotoxins that are generated during the ovulatory process. Intensive egg-laying hens readily develop ovarian carcinomatosis. Indeed, elevated levels of potentially mutagenic 8-oxo-guanine adducts were detected in avian ovarian epithelial cells isolated from the apical surfaces and perimeters of pre-and postovulatory follicles, respectively. Internucleosomal DNA fragmentation indicative of apoptosis was evident in ovarian surface epithelial cells associated with the formative site of ovulation (stigma line) and regressive ruptured follicles. It is conceivable that a genetically altered progenitor cell with unrepaired DNA but not committed to death (i.e., a unifocal "escape") could give rise to a transformed phenotype. Hence, the high rate of ovarian cancer in egg-laying hens could be the consequence of genomic damages to the ovarian surface epithelium associated with incessant ovulations, thereby increasing the likelihood of mutation and clonal expansion.

Key Words: ovulation • ovarian surface epithelium • oxo-guanine • apoptosis • ovarian cancer • hen

This article has been cited by other articles:

				D. A. Symonds, I. Merchenthaler, and J. A. Flaws Methoxychlor and Estradiol Induce Oxidative Stress DNA Damage in the Mouse Ovarian Surface Epithelium Toxicol. Sci., September 1, 2008; 105(1): 182 - 187. [Abstract] [Full Text] [PDF]

				J. E. Burdette, R. M. Oliver, V. Ulyanov, S. M. Kilen, K. E. Mayo, and T. K. Woodruff Ovarian Epithelial Inclusion Cysts in Chronically Superovulated CD1 and Smad2 Dominant-Negative Mice Endocrinology, August 1, 2007; 148(8): 3595 - 3604. [Abstract] [Full Text] [PDF]

				J. R. Giles, L. M. Olson, and P. A. Johnson Characterization of Ovarian Surface Epithelial Cells from the Hen: A Unique Model for Ovarian Cancer Experimental Biology and Medicine, December 1, 2006; 231(11): 1718 - 1725. [Abstract] [Full Text] [PDF]

				J. E. Burdette, S. J. Kurley, S. M. Kilen, K. E. Mayo, and T. K. Woodruff Gonadotropin-Induced Superovulation Drives Ovarian Surface Epithelia Proliferation in CD1 Mice Endocrinology, May 1, 2006; 147(5): 2338 - 2345. [Abstract] [Full Text] [PDF]

				W. J. Murdoch Carcinogenic Potential of Ovulatory Genotoxicity Biol Reprod, October 1, 2005; 73(4): 586 - 590. [Abstract] [Full Text] [PDF]