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ORIGINAL RESEARCH ARTICLE

In Vivo Hyperoxic Preconditioning Prevents Myocardial Infarction by Expressing Bcl-2

Hong Choi^*,1, Sang-Hyun Kim^,1, Yang-Sook Chun, Young-Suk Cho^*, Jong-Wan Park^* and Myung-Suk Kim^*,2

^* Department of Pharmacology, Department of Internal Medicine, and Department of Physiology, Seoul National University College of Medicine and Heart Research Institute SNUMRC, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea

To whom requests for reprints should be addressed at ² Department of Pharmacology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea. E-mail: kimmsu{at}snu.ac.kr

Preconditioning with oxidative stress has been demonstrated in vitro to stimulate the cellular adaptation to subsequent severe oxidative stress. However, it is uncertain whether this preconditioning works in vivo. In the present study, we examined in vivo the beneficial effect of oxidative preconditioning. After rats were pretreated with whole-body hyperoxygenation (100% O₂ at 3 atmosphere for 20 mins, four cycles with 20-min intermission), isolated hearts were subjected to 45-min ischemia followed by 90-min reperfusion. This hyperoxic preconditioning significantly reduced infarct size, cytochrome-c release, DNA fragmentation, and terminal deoxynucleotidyl transferase-mediated dUTD nick-end labeling-positive cell frequency in the left ventricle, biphasically with an early (30-min) and a delayed (48-hr) effect after the hyperoxygenation. Mechanistically, the NF-B activity and Bcl-2 expression were enhanced in the hearts, and a NF-B inhibitor, pyrrolidine dithiocarbamate, abolished the Bcl-2 induction as well as the infarct-limiting effect. An antioxidant, N-acetylcysteine, and protein kinase C (PKC) inhibitors chelerythrine and Gö 6983 also blocked the preconditioning effects. These results indicate that hyperoxia induces myocardial tolerance against ischemia-reperfusion injury in association with Bcl-2 induction by NF-B activation through reactive oxygen species and PKC-dependent signaling pathway.

Key Words: hyperoxia • preconditioning • reperfusion injury • heart • NF-B • Bcl-2

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