HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Masuzawa, K. Articles by Goto, K. Search for Related Content PubMed PubMed Citation Articles by Masuzawa, K. Articles by Goto, K.

---

EYE

A Model of Retinal Ischemia-Reperfusion Injury in Rats by Subconjunctival Injection of Endothelin-1

Koichi Masuzawa^*, Subrina Jesmin, Seiji Maeda, Yuichi Kaji, Tetsuro Oshika, Sohel Zaedi, Nobutake Shimojo, Naoko Yaji, Takashi Miyauchi^,1 and Katsutoshi Goto^*

^* Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan; Cardiovascular Division, Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan; Department of Ophthalmology, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan; and Department of Ophthalmology, Tokyo Women’s Medical University, Tokyo, Japan

To whom requests for reprints should be addressed at ¹ Cardiovascular Division, Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan. E-mail: t-miyauc{at}md.tsukuba.ac.jp

Abstract

The retinal ischemia-reperfusion model is used in the study of transient ischemia–related diseases, such as central retinal artery occlusion, angle-closure glaucoma, and others. There are two methods for experimentally producing an ischemia-reperfusion model in the rat retina: (i) the intraocular pressure is greatly raised by increasing the height of the infusion bottle connected with the needle in the anterior chamber; or (ii) the blood vessel that accompanies the optic nerve in retina is ligated. However, each method has some drawbacks. For example, in the first method, the needle must be fixed in the anterior chamber for 1 hr, thus, the technique is not stable and mechanical damage to ocular structures sometimes occurs. In the second method, because of the unavoidable involvement of the optic nerve, damage to the nerve induces retinal changes unrelated to ischemia. In this study, we injected endothelin (ET)-1 under the conjunctiva of the eyeball (subconjunctival injection), and evaluated whether a retinal ischemia-reperfusion model could be generated by this method, simply and noninvasively. We injected 4 x 10^–5 M ET-1 solution into the right eye of the rat and injected a control vehicle (artificial tears) into the left eye. From 5–60 mins after the injection, 50 mg/ml fluorescein isothiocyanate (FITC)-dextran was injected to the left ventricle of heart. Then, the retina was removed and flat mounted. We compared the perfusion conditions of the FITC-dextran to each retina in the right and left eye. There was a complete perfusion of FITC-dextran in the retinal main artery, vein, and the capillary vessels in all of the control eyes. However, perfusion could not be completely observed in the ET-1 injected eye from 5–35 mins after injection; afterwards, the flow was returned. This method of subconjunctival injection of ET-1 is, thus, a feasible technical option for producing a retinal ischemia-reperfusion model in rat.

Key Words: ischemia-reperfusion injury • endothelin-1 • subconjunctival injection • retina • rat

This article has been cited by other articles:

				D. V. Pow and C. M. Diaz AMD-like Lesions in the Rat Retina: A Latent Consequence of Perinatal Hemorrhage Invest. Ophthalmol. Vis. Sci., July 1, 2008; 49(7): 2790 - 2798. [Abstract] [Full Text] [PDF]