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Experimental Biology and Medicine 231:709-712 (2006)
© 2006 Society for Experimental Biology and Medicine

BASIC BIOLOGY

cDNA Cloning and Sequence Analysis of Preproendothelin-1 (PPET-1) from Salmon, Oncorhynchus keta

Hongyu Wang*, Jiexia Quan*, Ei-Ichi Kotake-Nara*,{dagger}, Tsuyoshi Uchide{ddagger}, Tadashi Andoh§ and Kaname Saida,1

* Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, 305-8566 Japan; {dagger} New Energy and Industrial Technology Development Organization, Kawasaki, 212-8544 Japan; {ddagger} Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, 034-8628 Japan; § Hokkaido National Fisheries Research Institute, Fisheries Research Agency, Kushiro, 085-0802 Japan; and Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Ikeda, 536-8577 Japan

To whom requests for reprints should be addressed at 1 Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. E-mail: k.saida{at}aist.go.jp

Abstract

The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively).

Key Words: salmon • endothelin-1 • stomach • cDNA • RACE (rapid amplification of cDNA ends) • precursor




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K. A. Hyndman and D. H. Evans
Endothelin and endothelin converting enzyme-1 in the fish gill: evolutionary and physiological perspectives
J. Exp. Biol., December 15, 2007; 210(24): 4286 - 4297.
[Abstract] [Full Text] [PDF]


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