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Intrapericardial Angiotensin II Stimulates Endothelin-1 and Atrial Natriuretic Peptide Formation of the In Situ Dog Heart

Ildikó Toma^*, Balázs Sax^*, Andrea Nagy^*, László Entz, Jr.^*, Miklós Rusvai, Alexander Juhász-Nagy^* and Violetta Kékesi^*,1

^* Department of Cardiovascular Surgery, Semmelweis University; and Department of Pathology and Forensic Veterinary Medicine, Szent István University, Budapest, Hungary

To whom requests for reprints should be addressed at ¹ Department of Cardiovascular Surgery, Semmelweis University, Városmajor u. 68, Budapest, Hungary, H-1122. E-mail: violetta_kekesi{at}yahoo.com

Abstract

Angiotensin (AT) II, endothelin (ET)-1, and atrial natriuretic peptide (ANP) play an important role in cardiovascular regulatory processes under physiologic and pathophysiologic conditions. All of these agents are present in the pericardial fluid, and alteration of their pericardial concentrations mirror changes in the myocardial interstitium. Moreover, the composition the pericardial fluid may also reflect the myocardial interaction of these agents. The local myocardial effects of AT II on cardiac ET-1 and ANP production, as well as on cardiovascular function, was studied by intrapericardial (ip) administration of AT II (0.125–1.0 µg/kg) to the in situ dog heart (n = 8). Big ET, ET-1, and ANP [1–28] fragment concentrations were measured by enzyme-linked immunosorbent assay in pericardial infusate samples and in peripheral blood before and after an AT II treatment of 15 mins. Systemic blood pressure (BP), heart rate (HR), and left ventricular contractility (dP/dt) were also recorded. In our studies, the pericardial big ET (but not ET-1) concentration was increased to a maximum value of 139 ± 28 versus 74 ± 12 pg/ml (control; P < 0.02) with ip AT II administration, with parallel elevations of the pericardial ANP levels (36.8 ± 7.2 vs. 24.4 ± 3.6 ng/ml; P < 0.05). The ip administration of AT II did not influence HR, and it elicited moderate changes in BP (BP_max, +14 ± 2 mm Hg, P < 0.001; dP/dt_max, +10 ± 3%, P < 0.02). The plasma levels of big ET, ET-1, and ANP did not change significantly. The results suggest that AT II promotes production of big ET and ANP in the heart. However, no detectable conversion of big ET-1 to ET-1 was observed within 15 mins. The myocardial formation of big ET-1 and ANP occurred, at least in part, independently of the changes in cardiovascular function.

Key Words: cardiovascular physiology • angiotensin II • endothelin-1 • atrial natriuretic peptide • pericardium

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