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HEART

Time Course Alterations of Myocardial Endothelin-1 Production During the Formation of Exercise Training–Induced Cardiac Hypertrophy

Motoyuki Iemitsu^*,, Seiji Maeda^*,,1, Takeshi Otsuki^*, Katsutoshi Goto and Takashi Miyauchi^*,

^* Center for Tsukuba Advanced Research Alliance, Institute of Health and Sport Sciences, Department of Pharmacology, Institute of Basic Medical Sciences, and Cardiovascular Division, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan

To whom requests for reprints should be addressed at ¹ Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan. E-mail: smaeda{at}tara.tsukuba.ac.jp

Abstract

Endothelin (ET)-1 is produced by endothelial cells and cardiac myocytes. ET-1 has positive inotropic and chronotropic effects on the heart and causes myocardial cell hypertrophy. Exercise training induces a physiologic cardiac hypertrophy. To study whether myocardial ET-1 is involved in the formation of exercise training–induced cardiac hypertrophy, we investigated time-course alterations of myocardial ET-1 gene expression and ET-1 peptide level in the heart of rats during a formative process of exercise training–induced cardiac hypertrophy. We used the hearts of rats that had been exercise-trained for 4 weeks (4WT) or 8 weeks (8WT) and sedentary control rats for 4 weeks (4WC) or 8 weeks (8WC). Exercise-trained rats performed treadmill running for 5 days/week (60 mins/day). Left ventricular mass index and wall thickness and stroke volume index, measured using echocardiography, in the 8WT group were significantly greater than in the 8WC group, although there were no differences between the 4WC and 4WT groups in these parameters. These results indicated that the 8WT rats developed physiologic cardiac hypertrophy, whereas the 4WT rats did not yet have cardiac hypertrophy. Myocardial ET-1 gene expression and tissue ET-1 concentration in the heart were significantly higher in the 8WT group than in the 8WC group, whereas these values did not differ between the 4WC and 4WT groups. The present study suggests that an alternation of myocardial ET-1 production corresponds with the formation of exercise training–induced cardiac hypertrophy. Therefore, the exercise training–induced change in myocardial ET-1 production may participate in a mechanism of exercise training–induced cardiac adaptation (e.g., cardiac hypertrophy).

Key Words: heart • treadmill • rat • cardiac adaptation