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Experimental Biology and Medicine 232:52-57 (2007)
© 2007 Society for Experimental Biology and Medicine

ORIGINAL RESEARCH ARTICLE

Cordyceps sinensis Mycelium Extract Induces Human Premyelocytic Leukemia Cell Apoptosis Through Mitochondrion Pathway

Qiao Xia Zhang1 and Jian Yong Wu2

Department of Applied Biology and Chemical Technology and the State Key Lab of Chinese Medicine and Molecular Pharmacology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong

To whom requests for reprints should be addressed at 2 Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. E-mail: bcjywu{at}polyu.edu.hk

This study was to identify the signaling pathways for the induction of HL-60 cell apoptosis by Cordyceps sinensis mycelium extract (CSME). CSME at 25 µg/ml induced nuclear fragmentation and DNA degradation, two hallmark events of apoptosis, in the HL-60 cells within 12–24 hrs of treatment. Concomitantly, several major events in the mitochondrial signal pathway occurred, including the loss of MTP ({Delta}{Psi}m), cytochrome c release into the cytoplasm, the decrease in Bcl-2 protein level, the translocation of Bax protein from cytoplasm into mitochondria, and the activation of caspase-2, –3, and –9, but caspase-8, the initiator caspase in the death receptor pathway, was not activated. These results suggest that CSME induces apoptosis in HL-60 cell through the mitochondrial pathway rather than the death receptor pathway.

Key Words: Cordyceps sinensis • HL-60 cell • caspases • mitochondria • cytochrome c • Bcl-2 proteins






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