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Experimental Biology and Medicine 232:385-389 (2007)
© 2007 Society for Experimental Biology and Medicine

ORIGINAL RESEARCH ARTICLE

A BRIEF COMMUNICATION

Copper Inhibition of Hydrogen Peroxide–Induced Hypertrophy in Embryonic Rat Cardiac H9c2 Cells

Yang Zhou*, Youchun Jiang{dagger} and Y. James Kang*,{dagger},1

* Department of Pharmacology and Toxicology; and {dagger} Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky 40202

To whom requests for reprints should be addressed at 1 Department of Medicine, University of Louisville School of Medicine, 511 S. Floyd Street, MDR 530, Louisville, KY 40202. E-mail: yjkang01{at}louisville.edu

Previous studies have shown that dietary copper deficiency causes cardiac hypertrophy and depression of vascular epithelial growth factor (VEGF) expression in mouse model. Copper replenishment in the diet reverses cardiac hypertrophy and restores VEGF expression. The present study was undertaken to specifically determine the role of VEGF in copper effect on cell hypertrophy. Embryonic rat cardiac H9c2 cells were exposed to hydrogen peroxide to develop hypertrophy, determined by increases in cell size and total protein content. Copper addition at 5 µM in cultures suppressed cell hypertrophy. In the presence of anti-VEGF antibody, copper inhibitory effect on cell hypertrophy was blunted, and VEGF alone mimicked the inhibitory effect of copper. The results thus demonstrated that VEGF is critically involved in copper inhibition of cell hypertrophy induced by hydrogen peroxide in the H9c2 cells.

Key Words: copper • hypertrophy • VEGF • protein synthesis • H9c2 cells






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