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Experimental Biology and Medicine 232:532-541 (2007)
© 2007 Society for Experimental Biology and Medicine

ORIGINAL RESEARCH ARTICLE

Membrane Lipid Composition of Pancreatic AR42J Cells: Modification by Exposure to Different Fatty Acids

Nama’a Audi*, María D. Mesa{dagger}, María A. Martínez*, Emilio Martínez-Victoria*, Mariano Mañas* and María D. Yago*,1

* Department of Physiology, Institute of Nutrition and Food Technology, University of Granada, Granada, Spain; and {dagger} Department of Biochemistry and Molecular Biology, Institute of Nutrition and Food Technology, University of Granada, Granada, Spain

To whom requests for reprints should be addressed at 1 Institute of Nutrition and Food Technology, C/Ramón y Cajal 4, 18071 Granada, Spain. E-mail: mdyago{at}ugr.es

Dietary fat type influences fatty acids in rat pancreatic membranes, in association with modulation of secretory activity and cell signalling in viable acini. We aimed to confirm whether AR42J cells are a valid model to study the interactions between lipids and pancreatic acinar cell function. For this purpose we have (i) compared the baseline fatty acid composition of AR42J cells with that of pancreatic membranes from rats fed a standard chow; (ii) investigated if fatty acids in AR42J membranes can be modified in culture; and (iii) studied if similar compositional variations that can be evoked in rats when dietary fat type is altered occur in AR42J cells. Weaning Wistar rats were fed for 8 weeks either a commercial chow (C) or semi-purified diets containing virgin olive oil (VOO) or sunflower oil (SO) as fat source. AR42J cells were incubated for 72 hrs in medium containing unmodified fetal calf serum (FCS, AR42J-C cells), FCS enriched with 18:1 n-9 (AR42J-O cells), or FCS enriched with 18:2 n-6 (AR42J-L cells). Fatty acids in crude membranes from rat pancreas and AR42J cells were determined by gas–liquid chromatography. Differences in membrane fatty acids between C rats and AR42J-C cells can be explained in part by variations in the amount of fatty acids in the extracellular environment. Supplementation of FCS with 18:1 n-9 or 18:2 n-6 changed the fatty acid spectrum of AR42J cells in a manner that resembles the pattern found, respectively, in VOO and SO rats, although AR42J-L cells were unable to accumulate 20:4 n-6. The AR42J cell line can be a useful tool to assess the effect of membrane compositional changes on acinar cell function. However, differences in baseline characteristics, and perhaps fatty acid metabolism, indicate that results obtained in AR42J cells should be confirmed with experiments in the whole animal.

Key Words: dietary fat • virgin olive oil • sunflower oil • AR42J • rat pancreas • cell model • membrane fatty acids • oleic acid • linoleic acid






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Copyright © 2007 by the Society for Experimental Biology and Medicine.