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ORIGINAL RESEARCH ARTICLE

Human Single-Chain Fv Antibodies Against Burkholderia mallei and Burkholderia pseudomallei

Department of Environmental and Infectious Disease Sciences, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306

To whom requests for reprints should be addressed at ¹ AFIP, Bldg. 54, Room 3025, 6825 16th Street, NW, Washington, DC 20306. E-mail: los{at}afip.osd.mil

Much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with Burkholderia mallei and Burkholderia pseudomallei for diagnostic and/or therapeutic purposes. Our present study focused on the screening of a phage-displayed nonimmune human single-chain Fv (scFv) antibody library against heat-killed B. mallei and B. pseudomallei for the generation of human scFv antibodies specific to the two pathogenic species of bacteria. Using two different panning procedures, we obtained seven different scFv phage antibodies that interacted with the heat-killed whole bacterial cells of B. mallei and B. pseudomallei. Our results demonstrate that panning of a human scFv antibody library against heat-killed whole bacterial cells may provide a valuable strategy for developing human monoclonal antibodies against the highly pathogenic bacteria.

Key Words: single-chain Fv • scFv • Burkholderia mallei • Burkholderia pseudomallei