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Experimental Biology and Medicine 232:881-886 (2007)
© 2007 Society for Experimental Biology and Medicine

ORIGINAL RESEARCH ARTICLE

DNA Methylation Is Not Likely to Be Responsible for hTERT Expression in Premalignant Cervical Lesions

Pagona Oikonomou*, Ioannis Messinis{dagger} and Aspasia Tsezou*{ddagger},1

* Departments of Biology, {dagger} Gynecology and Obstetrics, and {ddagger} Laboratory of Cytogenetics and Medical Genetics, Medical School, University of Thessalia, Larissa, Greece

To whom requests for reprints should be addressed at 1 Department of Biology, Medical School, University of Thessalia, 22 Papakyriazi str, 41 222 Larisa, Greece. E-mail: atsezou{at}med.uth.gr

Human telomerase reverse transcriptase (hTERT) mRNA expression seems to play an important role in cervical carcinogenesis. Analysis of the hTERT promoter region revealed the presence of a CpG island and a high overall GC content, suggesting a possible role for methylation in the regulation of hTERT gene expression. The present study was designed to evaluate the role of hTERT promoter methylation and E6/E7 human papilloma virus 16 (HPV-16) mRNA expression in hTERT regulation in premalignant cervical specimens. The methylation status of the hTERT promoter gene and hTERT mRNA quantification were investigated in 26 normal and 64 specimens of abnormal cytology using the MethyLight technique, Telo-TAGGG hTERT Quantification Kit and LightCycler technology. E6/E7 HPV-16 mRNA expression was also evaluated. No significant correlations were observed between hTERT mRNA expression and hTERT promoter methylation, as well as between telomerase activity and hTERT promoter methylation in normal and in premalignant cervical specimens. E6/E7 HPV-16 mRNA expression was observed in 72% of HPV-16–infected samples and was correlated with hTERT mRNA expression and telomerase activity (P < 0.05). This is the first study investigating the role of hTERT promoter methylation in hTERT mRNA expression and telomerase activity in premalignant lesions. The observed lack of correlation suggests that other mechanisms might be involved in the regulation of hTERT expression. The correlation between hTERT mRNA and E6/E7 mRNA expression confirms the role of HPV infection in hTERT regulation.

Key Words: DNA promoter methylation • telomerase • hTERT • cervical lesions






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