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ORIGINAL RESEARCH ARTICLE

Topical Application of Plasma Fibronectin in Full-Thickness Skin Wound Healing in Rats

A-Hon Kwon^*,1, Zeyu Qiu^* and Yutaka Hirao

^* Department of Surgery, Kansai Medical University, Osaka, Japan; and Research & Development Division, Hirakata Laboratory, Benesis Corporation, Osaka, Japan

To whom requests for reprints should be addressed at ¹ Department of Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, Osaka, 570-8507, Japan. E-mail: kon{at}takii.kmu.ac.jp

Fibronectin (Fn) has been shown to play an important role in wound healing because it appears to be the stimulus for migration of fibroblasts and epidermal cells. The purpose of this study was to investigate whether topical application of plasma Fn (pFn) improves healing of full-thickness skin wounds in rats. A round section of full-thickness skin (diameter of approximately 15 mm) was resected in rats. Animals were then divided into two groups, and wounds were treated topically with a single application of human plasma albumin (control group) or human pFn (FN group). Wound closure rate, hydroxyproline concentration, and histologic features (immunohistochemical staining) were evaluated. The FN group had a significantly higher wound closure rate and hydroxyproline level in the skin than the control group. Histologic analysis of macrophage and fibroblast migration, collagen regeneration, and epithelialization were significantly increased in the FN group compared with the control group. A single topical application of pFn increased the migration of macrophages, myofibroblasts, and fibroblasts. Moreover, further release of transforming growth factor-ß1 from activated fibroblasts, keratinocytes, and epithelial cells may also contribute to the beneficial effect of pFn on wound healing.

Key Words: fibronectin • wound healing • transforming growth factor-ß1 • fibroblast • hydroxyproline

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