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First published online August 14, 2008
Experimental Biology and Medicine 233:1315-1322 (2008)
doi: 10.3181/0802-RM-68
© 2008 by the Society for Experimental Biology and Medicine
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ORIGINAL RESEARCH ARTICLE

Acetaminophen Is Cardioprotective Against H2O2-Induced Injury In Vivo

Kathryn M. Jaques-Robinson, Roseli Golfetti, Sunanda S. Baliga, Norell M. Hadzimichalis and Gary F. Merrill1

Division of Life Sciences, Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854

To whom requests for reprints should be addressed at 1 Division of Life Sciences, Department of Cell Biology and Neuroscience, Rutgers University, 604 Allison Rd., Piscataway, NJ 08854. E-mail: merrill{at}biology.rutgers.edu

Here we report our ongoing investigation of the cardiovascular effects of acetaminophen, with emphasis on oxidation-induced canine myocardial dysfunction. The objective of the current study was to investigate whether acetaminophen could attenuate exogenous H2O2-mediated myocardial dysfunction in vivo. Respiratory, metabolic, and hemodynamic indices such as left ventricular function (LVDP and ±dP/dtmax), and percent ectopy were measured in anesthetized, open-chest dogs during intravenous administration of 0.88 mM, 2.2 mM, 6.6 mM H2O2. Following 6.6 mM H2O2, tissue from the left ventricle was harvested for electron microscopy. Left ventricular function did not vary significantly between vehicle and acetaminophen groups under baseline conditions. Acetaminophen-treated dogs regained a significantly greater fraction of baseline function after high concentrations of H2O2 than vehicle-treated dogs. Moreover, the incidence of H2O2-induced ventricular arrhythmias was significantly reduced in the acetaminophen-treated group. Percent ectopy following 6.6 mM concentrations of H2O2 was 1 ± 0.3 vs. 0.3 ± 0.1 (P < 0.05) for vehicle- and acetaminophen-treated dogs, respectively. Additionally, electron micrograph images of left ventricular tissue confirmed preservation of tissue ultrastructure in acetaminophen-treated hearts when compared to vehicle. We conclude that, in the canine myocardium, acetaminophen is both functionally cardioprotective and antiarrhythmic against H2O2-induced oxidative injury.

Key Words: canine myocardium • ROS • oxidative stress • pathology • ischemia/reperfusion injury






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