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First published online April 11, 2008
Experimental Biology and Medicine 233:689-693 (2008)
doi: 10.3181/0710-RM-279
© 2008 by the Society for Experimental Biology and Medicine
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ORIGINAL RESEARCH ARTICLE

Expression of {alpha}-Actinin-1 in Human Glomerular Mesangial Cells In Vivo and In Vitro

Chen Yang*,1,2 and William F. Glass, II*,{dagger}

* Department of Pathology and Anatomy, Eastern Virginia Medical School, Norfolk, Virginia 23501; and {dagger} Nephrocor and Bostwick Laboratories, Richmond, Virginia 23060

To whom requests for reprints should be addressed at 1 8701 Watertown Plank Road, Milwaukee, WI 53226. E-mail: chenyang{at}mcw.edu

Recent studies have demonstrated important roles of {alpha}-actinins in glomerular disease, while little information is known about the expression profile of {alpha}-actinins in human glomerular mesangial cells. Here, immunofluorescence and confocal microscopy showed that {alpha}-actinin-1 exclusively distributed along mesangial cells in human glomeruli of IgA nephropathy. RT-PCR and Western blot further confirmed the expression of {alpha}-actinin-1 in primary cultured human mesangial cells. We also found that transforming growth factor-β 1 (TGF-β 1) stimulated ACTN1 gene transcription and that transiently transfected {alpha}-actinin-1 significantly increased TGF-β 1-induced plasminogen activator inhibitor-1 (PAI-1) promoter activity in human mesangial cells. These findings suggest that {alpha}-actinin-1 may play a role in human glomerular disease.

Key Words: {alpha}-actinin-1 • mesangial cells • transforming growth factor-β1 • plasminogen activator inhibitor-1 • glomerular disease






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