HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 233/7/897    most recent 0802-RM-43v1 Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hofer, M. Articles by Vacek, A. Search for Related Content PubMed PubMed Citation Articles by Hofer, M. Articles by Vacek, A.

---

ORIGINAL RESEARCH ARTICLE

Homeostatic Action of Adenosine A₃ and A₁ Receptor Agonists on Proliferation of Hematopoietic Precursor Cells

Michal Hofer^*,1, Milan Pospíil^*, Vladimír Znojil, Jiina Holá^*, Denisa treitová^* and Antonín Vacek^*

^* Laboratory of Experimental Hematology, Institute of Biophysics, v.v.i., Academy of Sciences of the Czech Republic, Brno, Czech Republic; and Institute of Pathological Physiology, Medical Faculty, Masaryk University, Brno, Czech Republic

To whom requests for reprints should be addressed at ¹ Laboratory of Experimental Hematology, Institute of Biophysics, v.v.i., Academy of Sciences of the Czech Republic, Královopolská 135, CZ-61265 Brno, Czech Republic. E-mail: hofer{at}ibp.cz

Two adenosine receptor agonists, N⁶-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) and N⁶-cyclopentyladenosine (CPA), which selectively activate adenosine A₃ and A₁ receptors, respectively, were tested for their ability to influence proliferation of granulocytic and erythroid cells in femoral bone marrow of mice using morphological criteria. Agonists were given intraperitoneally to mice in repeated isomolar doses of 200 nmol/kg. Three variants of experiments were performed to investigate the action of the agonists under normal resting state of mice and in phases of cell depletion and subsequent regeneration after treatment with the cytotoxic drug 5-fluorouracil. In the case of granulopoiesis, IB-MECA 1) increased by a moderate but significant level proliferation of cells under normal resting state; 2) strongly increased proliferation of cells in the cell depletion phase; but 3) did not influence cell proliferation in the regeneration phase. CPA did not influence cell proliferation under normal resting state and in the cell depletion phase, but strongly suppressed the overshooting cell proliferation in the regeneration phase. The stimulatory effect of IB-MECA on cell proliferation of erythroid cells was observed only when this agonist was administered during the cell depletion phase. CPA did not modulate erythroid proliferation in any of the functional states investigated, probably due to the lower demand for cell production as compared with granulopoiesis. The results indicate opposite effects of the two adenosine receptor agonists on proliferation of hematopoietic cells and suggest the plasticity and homeostatic role of the adenosine receptor expression.

Key Words: adenosine receptors • hematopoiesis • cell proliferation

This article has been cited by other articles:

				M. Katebi, M. Soleimani, and B. N. Cronstein Adenosine A2A receptors play an active role in mouse bone marrow-derived mesenchymal stem cell development J. Leukoc. Biol., March 1, 2009; 85(3): 438 - 444. [Abstract] [Full Text] [PDF]