HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 233/9/1088    most recent 0712-RM-337v1 Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sarada, S. Articles by Ilavazhagan, G. Search for Related Content PubMed PubMed Citation Articles by Sarada, S. Articles by Ilavazhagan, G.

---

ORIGINAL RESEARCH ARTICLE

Role of Oxidative Stress and NFkB in Hypoxia-Induced Pulmonary Edema

Department of Experimental Biology, Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi, India

To whom requests for reprints should be addressed at ¹ Department of Experimental Biology, Defense Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi-54, India. E-mail: saradasks{at}gmail.com

Hypoxia is well known to increase the free radical generation in the body, leading to oxidative stress. In the present study, we have determined whether the increased oxidative stress further upregulates the nuclear transcription factor (NFkB) in the development of pulmonary edema. The rats were exposed to hypobaric hypoxia at 7620 m (280 mm Hg) for different durations, that is, 3 hrs, 6 hrs, 12 hrs, and 24 hrs at 25 ± 1°C. The results revealed that exposure of animals to hypobaric hypoxia led to a significant increase in vascular leakage, with time up to 6 hrs (256.38 ± 61 rfu/g) as compared with control (143.63 ± 60.1 rfu/g). There was a significant increase in reactive oxygen species, lipid peroxidation, and superoxide dismutase levels, with a concurrent decrease in lung glutathione peroxidase activity. There was 13-fold increase in the expression of NFkB level in nuclear fraction of lung homogenates of hypoxic animals over control rats. The DNA binding activity of NFkB was found to be increased significantly (P < 0.001) in the lungs of rats exposed to hypoxia as compared with control. Further, we observed a significant increase in proinflammatory cytokines such as IL-1, IL-6, and TNF- with concomitant upregulation of cell adhesion molecules such as ICAM-I, VCAM-I, and P-selectin in the lung of rats exposed to hypoxia as compared with control. Interestingly, pretreatment of animals with curcumin (NFkB blocker) attenuated hypoxia-induced vascular leakage in lungs with concomitant reduction of NFkB levels. The present study therefore reveals the possible involvement of NFkB in the development of pulmonary edema.

Key Words: HAPE • NFkB • oxidative stress • proinflammatory cytokines

This article has been cited by other articles:

				Z. Song, K. A. Harris, and B. T. Thach Laryngeal constriction during hypoxic gasping and its role in improving autoresuscitation in two mouse strains J Appl Physiol, April 1, 2009; 106(4): 1223 - 1226. [Abstract] [Full Text] [PDF]