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Regular Manuscript

Effect of Buddleja officinalis on high glucose-induced vascular inflammation in human umbilical vein endothelial cells

¹ Professional Graduate School of Oriental Medicine
² Korea Institute of Oriental Medicine

^* To whom correspondence should be addressed. E-mail: shrons{at}wonkwang.ac.kr.

	Abstract

In this study, we aimed to investigate whether an aqueous extract of Buddleja officinalis (ABO) suppresses high glucose-induced vascular inflammatory process in the primary cultured human umbilical vein endothelial cells (HUVEC). The increase of cell adhesion molecules (CAMs) expression such ICAM-1, VCAM-1, and E-selectin by high glucose was significantly attenuated by pretreatment with ABO in a dose-dependent manner. Enhanced cell adhesion by high glucose in co-cultured U937 and HUVEC induced was also blocked by pretreatment with ABO. Pretreatment with ABO blocked high glucose-induced ROS formation. In addition, ABO suppressed the transcriptional activity of NF-B and I-B phosphorylation under high glucose condition. Pretreatment with N(G)-nitro-L-arginine methyl ester (L-NAME), an endothelial NO inhibitor, attenuated the protective action of ABO on high glucose-induced CAMs expression, suggesting a potential role of NO signaling. The present data suggested that ABO could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of ROS and NF-B activation in HUVEC.

Key Words: Buddleja officinalis, Adhesion molecules, ROS, NF-B, HUVEC