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First published online April 29, 2008
Experimental Biology and Medicine doi: 10.3181/0712-RM-356
© 2008 by the Society for Experimental Biology and Medicine
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Regular Manuscript

Dissimilar differentiation potential of mesenchymal stem cells isolated from bone marrow, umbilical cord blood and adipose tissue

Carmen Rebelatto 1, Alessandra Aguiar 2, Mariana Moretão 2, Alexandra Senegaglia 1, Paula Hansen 1, Fabiane Barchiki 1, João Oliveira 1, Joana Martins 1, Crisciele Kuligovski 2, Fernanda Mansur 2, Andressa Christofis 1, Vivian Amaral 1, Paulo Brofman 1, Samuel Goldenberg 2, Lia Nakao 1, and Alejandro Correa 3*

1 Pontifícia Universidade Católica do Paraná
2 Instituto de Biologia Molecular do Paraná
3 Instituto de Biologia Molecular do Parana

* To whom correspondence should be addressed. E-mail: alejandro{at}tecpar.br.


  Abstract

Mesenchymal stem cells (MSCs) have been claimed to be promising candidates for use in new cell-based therapeutic strategies, such as mesenchyme-derived tissue repair. They are easily isolated from adult tissues and are not ethically restricted. MSC-related literature, however, is conflicting in relation to MSC differentiation potential and molecular markers. Here, we compared MSCs isolated from bone marrow (BM),, umbilical cord blood (UCB) and adipose tissue (AT). The isolation efficiency for both bone marrow (BM) and adipose tissue (AT) was 100%, but that from umbilical cord blood (UCB) was only 30%. MSCs from these tissues are morphologically and immunophenotypically similar even though their differentiation diverges. Differentiation to osteoblast and chondroblast was similar among MSCs from all sources, as analyzed by cytochemistry. Adipogenic differentiation showed that UCB-MSCs produced few and small lipid vacuoles in contrast to BM-MSCs and adipose tissue-derived stem cells (ADSCs) (arbitrary differentiation values of 245.57 ± 943 and 243.89 ± 145.52 µm2/nucleus, respectively). The mean area occupied by individual lipid droplets was 7.37 µm2 for BM-MSCs and 2.36 µm2 for ADSCs, indicating more mature adipocytes in BM-MSCs than ADSCs treated cultures. We analyzed FAPB4, osteonectin, ALP and type II collagen gene expression by qPCR to confirm adipogenic, osteogenic and chondrogenic differentiation, respectively. Results showed that all three sources presented a similar capacity for chondrogenic and osteogenic differentiation and they differ in their adipogenic potential. Therefore, it may be crucial to pre-determine the most appropriate MSC source for future clinical applications.

Key Words: mesenchymal stem cells, bone marrow, umbilical cord blood, adipose tissue, differentiation




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Copyright © 2008 by the Society for Experimental Biology and Medicine.