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ORIGINAL RESEARCH ARTICLE

Effects of 3,5-Diiodo-L-Thyronine Administration on the Liver of High Fat Diet-Fed Rats

Elena Grasselli^*, Laura Canesi^*, Adriana Voci^*, Rita De Matteis^,, Ilaria Demori^*, Emilia Fugassa^* and Laura Vergani^*,1

^* Dipartimento di Biologia, Università di Genova, Genova, Italy; Istituto di Scienze Fisiologiche, Università Carlo Bo, Urbino, Italy; and Istituto di Ricerca sull’Attività Motoria, Università Carlo Bo, Urbino, Italy

To whom requests for reprints should be addressed at ¹ Dipartimento di Biologia, Università di Genova, Corso Europa 26, 16132 Genova, Italy. E-mail: laura.vergani{at}unige.it

In rats fed a high fat diet (HFD), long-term administration of 3,5-diiodo-L-thyronine (T₂), a naturally occurring iodothyronine, was shown to reduce body-weight gain, fat mass, and hepatic lipid accumulation. This work was aimed at investigating the mechanisms of T₂ action in the liver of HFD rats. The results show that HFD induces liver lipid peroxidation and stimulates the activity of enzymes involved in hydrogen peroxide (H₂O₂) metabolism, catalase in particular. Moreover, quantitative RT-PCR revealed HFD-induced upregulation of the transcription factor PPAR, as well as of metallothionein isoforms (MT-1 and MT-2). T₂ administration prevented the HDF-induced lipid peroxidation, as well as the increase in H₂O₂ metabolism, and reduced the upregulation of both PPAR and MT-2. These data demonstrate that in the liver of HFD rats, T₂ prevents both lipid accumulation and oxidative stress associated with increased fat metabolism.

Key Words: Wistar rats • oxidative stress • lipid peroxidation • metallothioneins (MT-1 and MT-2)