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ORIGINAL RESEARCH ARTICLE

Effect of Buddleja officinalis on High-Glucose-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Yun Jung Lee^*,, Dae Gill Kang^*,1, Jin Sook Kim and Ho Sub Lee^*,,1

^* Professional Graduate School of Oriental Medicine, Iksan, Jeonbuk, 570-749, Republic of Korea; Medical Resources Research Institute, Wonkwang University, 570-749, Republic of Korea; and Korea Institute of Oriental Medicine, Daejeon, 305-811, Republic of Korea

To whom requests for reprints should be addressed at ¹ Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea. E-mail: dgkang{at}wku.ac.kr or host{at}wku.ac.kr

In this study, we aimed to investigate whether an aqueous extract of Buddleja officinalis (ABO) suppresses high-glucose-induced vascular inflammatory processes in the primary cultured human umbilical vein endothelial cells (HUVEC). The high-glucose-induced increase in expression of cell adhesion molecules (CAMs) such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and endothelial-selectin (E-selectin) was significantly attenuated by pretreatment with ABO in a dose-dependent manner. Enhanced cell adhesion caused by high glucose in co-cultured U937 and HUVEC was also blocked by pretreatment with ABO. Pretreatment with ABO also blocked formation of high-glucose-induced reactive oxygen species (ROS). In addition, ABO suppressed the transcriptional activity of NF-B and IB phosphorylation under high-glucose conditions. Pretreatment with N(G)-nitro-L-arginine methyl ester (L-NAME), an endothelial nitric oxide (NO) synthase inhibitor, attenuated the protective action of ABO on high-glucose-induced CAM expression, suggesting a potential role of NO signaling. The present data suggest that ABO could suppress high-glucose-induced vascular inflammatory processes, and ABO may be closely related with the inhibition of ROS and NF-B activation in HUVEC.

Key Words: Buddleja officinalis • adhesion molecules • ROS • NF-B • HUVEC